Part:BBa_K4773001:Design
RBS-NicB
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The expression of NicB may require the co-expression of other nicotine catabolic enzymes, such as NicA, NicC, NicD, and NicX, to achieve complete degradation of nicotine to fumarate and succinate. The expression of NicB may affect the growth and viability of the bacterial cells, due to the production of 6-hydroxy-3-succinoylpyridine and other toxic intermediates. Therefore, it may be necessary to optimize the culture conditions and/or provide detoxification mechanisms for the bacterial cells.
Source
This part is derived from the genomic sequence of Pseudomonas putida S16, a nicotine-degrading bacterium isolated from tobacco waste.
References
Tang H, Wang L, Wang W, Yu H, Zhang K, Yao Y, Xu P. Systematic unraveling of the unsolved pathway of nicotine degradation in Pseudomonas. PLoS Genet. 2013 Oct;9(10):e1003923. doi: 10.1371/journal.pgen.1003923. Epub 2013 Oct 31. PMID: 24204323; PMCID: PMC3814310
Wang L, Tang H, Wang S, Liu R, Liu M, Zhang Y, Chen T, He X, Li S, Xu P. Novel nicotine oxidoreductase-encoding gene involved in nicotine degradation by Pseudomonas putida strain S16. Appl Environ Microbiol. 2012 Jan;78(2):364-72. doi: 10.1128/AEM.06262-11. Epub 2011 Nov 4. PMID: 22057864; PMCID: PMC3255647