Translational_Unit

Part:BBa_K4773001:Design

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-09-27)


RBS-NicB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression of NicB may require the co-expression of other nicotine catabolic enzymes, such as NicA, NicC, NicD, and NicX, to achieve complete degradation of nicotine to fumarate and succinate. The expression of NicB may affect the growth and viability of the bacterial cells, due to the production of 6-hydroxy-3-succinoylpyridine and other toxic intermediates. Therefore, it may be necessary to optimize the culture conditions and/or provide detoxification mechanisms for the bacterial cells.


Source

This part is derived from the genomic sequence of Pseudomonas putida S16, a nicotine-degrading bacterium isolated from tobacco waste.

References

Tang H, Wang L, Wang W, Yu H, Zhang K, Yao Y, Xu P. Systematic unraveling of the unsolved pathway of nicotine degradation in Pseudomonas. PLoS Genet. 2013 Oct;9(10):e1003923. doi: 10.1371/journal.pgen.1003923. Epub 2013 Oct 31. PMID: 24204323; PMCID: PMC3814310

Wang L, Tang H, Wang S, Liu R, Liu M, Zhang Y, Chen T, He X, Li S, Xu P. Novel nicotine oxidoreductase-encoding gene involved in nicotine degradation by Pseudomonas putida strain S16. Appl Environ Microbiol. 2012 Jan;78(2):364-72. doi: 10.1128/AEM.06262-11. Epub 2011 Nov 4. PMID: 22057864; PMCID: PMC3255647